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While sequences With this databases are wholly included with the Refseq consultant genomes databases, it does not comprise the alternate loci and thus avoids sequence redundancy introduced by which includes alternate loci. This database is recommended if you are not thinking of versions represented by alternate loci.
Pools of pre-created primers will help generate complete expression profiles, In particular where by assay sensitivity is critically crucial.
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In distinction to random primers, oligo (dT) primers are usually not well suited for degraded samples but are fantastic for setting up cDNA libraries and recommended to be used with reverse transcriptases (RTs) as Superscript IV RT.
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Help Small complexity regions are some locations inside a DNA sequence which have biased more info base compositions for instance a extend of ACACACACACACACACACA. Internal hybridization oligo parameters
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